Two-photon imaging using adaptive phase compensated ultrashort laser pulses

作者：Xi, Peng; Andegeko, Yair; Pestov, Dmitry; Lovozoy, Vadim V; Dantus, Marcos^*

来源：Journal of Biomedical Optics, 2009, 14(1): 014002.

DOI：10.1117/1.3059629

• multiphoton microscopy
• cell imaging
• two-photon excitation fluorescence
• chromatic dispersion
• ultrashort pulse
• adaptive dispersion compensation

An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The delivery of broad-bandwidth (similar to 100 nm), sub-12-fs pulses was confirmed by interferometric autocorrelation measurements at the focal plane. A comparison of two-photon imaging with transform-limited and second-order-dispersion compensated laser pulses of the same energy showed a 6-to-11-fold improvement in the two-photon excitation fluorescence signal when applied to cells and tissue, and up to a 19-fold improvement in the second harmonic generation signal from a rat tendon specimen.

• 出版日期2009-2
• 单位上海交通大学