Detection of His-tagged Long-R-3-IGF-I in a black market product

作者：Kohler Maxie; Thomas Andreas; Walpurgis Katja; Terlouw Koen; Schaenzer Wilhelm; Thevis Mario^*

来源：Growth Hormone & IGF Research, 2010, 20(5): 386-390.

DOI：10.1016/j.ghir.2010.07.001

• Histidine-tag
• Orbitrap mass spectrometry
• Recombinant protein
• Doping
• Sport

Objective: Performance-enhancing substances are illicitly used in elite or amateur sports and may be obtained from the black market due to a cheaper and easier availability. Although various studies have shown that black market products frequently do not contain the declared substances, enormous amounts of illegally produced and/or imported drugs are confiscated from athletes or at customs with alarming results concerning the outcome of the analyses of the ingredients. This case report describes the identification of His-tagged Long-R-3-IGF-I, which is usually produced for biochemical studies, in an injection vial.
Design: The ingredients were isolated by immunoaffinity purification and identified by nano-UPLC, high-resolution/high accuracy mass spectrometry of the intact and trypsinated substance and by an enzyme-linked immunosorbent assay.
Results: (Tandem) mass spectra characterized the protein as Long-R-3-IGF-I with a His(6)-tag attached to the C-terminus by the linker amino acids Leu-Glu.
Conclusion: His-tags are commonly added to proteins during synthesis to allow a convenient and complete purification of the final product and His-tags are subsequently removed by specific enzymes when being attached to the N-terminus. The effects of His-tagged Long-R-3-IGF-I in humans have not been elucidated or described and the product may rather be a by-product from biochemical studies than synthesized for injection purposes.

• 出版日期2010-10