Anti-inflammatory properties and expression in selected organs of canine interleukin-1 beta splice variant 1

作者：Kiczak L^*; Walecka Zacharska E; Bania J; Sambor I; Stefaniak T; Dzigiel P; Zacharski M; Tomaszek A; Rybinska I; Paslawska U

来源：Veterinary Immunology and Immunopathology, 2015, 167(3-4): 91-95.

DOI：10.1016/j.vetimm.2015.07.007

• Dog
• Interleukin-1 beta
• Alternative splicing
• Liver
• Lung
• Inflamation

The IL-1 beta gene can be also be spliced with the intron 4 retention; the result is a IL-1 beta splice variant 1 (IL-1 beta sv1), which was significantly up-regulated in failing myocardium of dogs suffering from chronic degenerative valvular disease (CDVD). Expression of IL-1 beta sv1 was assessed, at both RNA and protein levels, in organs affected by heart failure, namely, kidneys, liver, and lungs from 35 dogs suffering chronic degenerative valvular disease (CDVD) and in 20 disease free control dogs. IL-1 beta sv1 RNA was detected in the dogs from both groups. In the CDVD group, the highest RNA and protein IL-1 beta sv1 levels were observed in lungs, followed, in that order, by the liver and kidneys. IL-1 beta sv1 protein was found in the cytoplasm of hepatocytes and IL-1 beta sv1-overexpressing DH82 cells. In lungs, IL-1 beta sv1 was localized in the cytoplasm and in the nuclei of bronchiolar epithelial and smooth-muscle cells. Cytoplasmic and nuclear IL-1 beta sv1 expression was observed in macrophages, and a strong nuclear signal was detected in epithelial cells of the alveolar sacs. Following lipopolysaccharide (LPS) stimulation, overexpression of IL-1 beta sv1 in DH82 cells decreased the pro-inflammatory response. Our results indicate that IL-1 beta sv1 is constitutively expressed in both normal tissues and in tissues from cases of heart failure. The presence of IL-1 beta sv1 in tissues exposed to invading agents and its anti-inflammatory activity in DH82 cells may point to its immunomodulatory role in vivo.

• 出版日期2015-10-15