Analysis of the sporicidal activity of chlorine dioxide disinfectant against Bacillus anthracis (Sterne strain)

作者：Chatuev B M; Peterson J W^*

来源：Journal of Hospital Infection, 2010, 74(2): 178-183.

DOI：10.1016/j.jhin.2009.09.017

• Bacillus anthracis
• Chlorine dioxide
• Disinfectant
• Sodium hypochlorite
• Spores

Routine surface decontamination is an essential hospital and laboratory procedure, but the list of effective, noncorrosive disinfectants that kill spores is limited. We investigated the sporicidal. potential of an aqueous chlorine dioxide solution and encountered some unanticipated problems. Quantitative bacteriological culture methods were used to determine the log(10) reduction of Bacillus anthracis (Sterne strain) spores following 3 min exposure to various concentrations of aqueous chlorine dioxide solutions at room temperature in seated tubes, as well. as spraying onto plastic and stainless steel. surfaces in a biological safety cabinet. Serial 10-fold dilutions of the treated spores were then plated on 5% sheep blood agar plates, and the survivor colonies were enumerated. Disinfection of spore suspensions with aqueous chlorine dioxide solution in seated microfuge tubes was highly effective, reducing the viable spore counts by 8 log(10) in only 3 min. By contrast, the process of spraying or spreading the disinfectant onto surfaces resulted in only a 1 log(10) kill because the chlorine dioxide gas was rapidly vaporised from the solutions. Full potency of the sprayed aqueous chlorine dioxide solution was restored by preparing the chlorine dioxide solution in 5% bleach (0.3% sodium hypochlorite). The volatility of chlorine dioxide can cause treatment failures that constitute a serious hazard for unsuspecting users. Supplementation of the chlorine dioxide solution with 5% bleach (0.3% sodium hypochlorite) restored full potency and increased stability for one week.

• 出版日期2010-2