Axonal Outgrowth and Dendritic Plasticity in the Cortical Peri-Infarct Area After Experimental Stroke

作者：Ueno Yuji; Chopp Michael; Zhang Li; Buller Benjamin; Liu Zhongwu; Lehman Norman L; Liu Xian Shuang; Zhang Yi; Roberts Cynthia; Zhang Zheng Gang^*

来源：Stroke, 2012, 43(8): 2221-U412.

DOI：10.1161/STROKEAHA.111.646224

• stroke
• axonal outgrowth
• high molecular weight neurofilament
• GSK-3 beta
• Akt

Background and Purpose-Axonal remodeling is critical to brain repair after stroke. The present study investigated axonal outgrowth after stroke and the signaling pathways mediating axonal outgrowth in cortical neurons. %26lt;br%26gt;Methods-Using a rodent model of middle cerebral artery occlusion, we examined high-molecular weight neurofilament (NFH) immunoreactive axons and myelin basic protein-positive oligodendrocytes in the peri-infarct area. In vitro, using cultured cortical neurons in a microfluidic chamber challenged by oxygen-glucose deprivation (OGD), we investigated mechanisms selectively regulating axonal outgrowth after OGD. %26lt;br%26gt;Results-NFH+ axons and MBP+ oligodendrocytes substantially increased in the peri-infarct area during stroke recovery, concomitantly with an increase in dendrites and spines identified by Golgi-Cox staining. In vitro, cortical neurons subjected to OGD exhibited significant increases in axonal outgrowth and in phosphorylated NFH protein levels, concurrently with downregulation of phosphatase tensin homolog deleted on chromosome 10, activation of Akt, and inactivation of glycogen synthase kinase-3 beta in regenerated axons. Blockage of phosphoinositide 3-kinase with pharmacological inhibitors suppressed Akt activation and attenuated phosphorylation of glycogen synthase kinase-3 beta, which resulted in suppression of phosphorylated NFH and axonal outgrowth after OGD; whereas GSK-3 inhibitors augmented axonal regeneration and elevated phosphorylated NFH levels after OGD. %26lt;br%26gt;Conclusions-Stroke induces axonal outgrowth and myelination in rodent ischemic brain during stroke recovery, and the phosphoinositide 3-kinase/Akt/glycogen synthase kinase-3 beta signaling pathway mediates axonal regeneration of cortical neurons after OGD. (Stroke. 2012; 43: 2221-2228.)

• 出版日期2012-8