Daily Intake of High-Fat Diet with Lysophosphatidic Acid-Rich Soybean Phospholipids Augments Colon Tumorigenesis in Kyoto Apc Delta Rats

作者：Tsutsumi Toshihiko^*; Inoue Manami; Okamoto Yoko; Ishihara Akira; Tokumura Akira

来源：Digestive Diseases and Sciences, 2017, 62(3): 669-677.

DOI：10.1007/s10620-016-4434-5

• Lysophosphatidic acid
• Colorectal cancer
• Azoxymethane/dextran sodium sulfate
• Dietary administration
• Tumor location

Oral administration of lysophosphatidic acid (LPA) was shown to attenuate gastric ulceration in rats and mice but aggravate intestinal tumorigenesis in mice.
The present study examined whether dietary LPA induces or prevents development of colorectal tumor in rats.
Kyoto Apc Delta rats fed high-fat diet with or without an LPA-rich soybean phospholipid mixture (LSP, 0.1 or 1%) were treated with azoxymethane and dextran sodium sulfate to induce colorectal tumorigenesis. Rats were killed 15 weeks after azoxymethane treatment, and size, total number, location, and severity of colorectal tumors were assessed. Expression of mRNA of LPA receptors in rat colon tissue was assayed.
Rats fed the diet supplemented with 1% LSP had a higher number of tumors 2-4 mm long compared than those with or without 0.1% LSP. The mean distance of tumors > 4 mm long from the anus was significantly higher than those of tumors < 2 and 2-4 mm long in rats fed 1% LSP-supplemented diet. Supplementation of the diet with 0.1% LSP decreased mRNA expression of LPA5 in colon tumors of rats.
Dietary supplementation of LPA-rich phospholipids dose-dependently augmented colorectal tumorigenesis. Decreased expression of LPA5 in colon tumors may be relevant to augmented tumorigenesis.

• 出版日期2017-3