A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA

作者：Funabashi Hisakage^*; Shigeto Hajime; Nakatsuka Keisuke; Kuroda Akio

来源：Analyst, 2015, 140(4): 999-1003.

DOI：10.1039/c4an02064b

A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.

• 出版日期2015