科研之友
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摘要
Objective: To investigate the protective mechanism of atorvastatin against cerebral I/R injury in mice. Methods: Mice were given 10 mg/kg/d atorvastatin for consecutive 3 d before middle cerebral artery occlusion and reperfusion (MCAO/R). At 48 h after MCAO/R, the infarct volume and brain water content were evaluated using TTC staining and standard wet-dry method. The effect of atorvastatin on cell apoptosis was detected by terminal-deoxynucleotidyl transferase mediated nick end labeling assay. The mRNA expression of matrix metalloproteinase (MMP)-2 and MMP-9 were detected by real-time polymerase chain reaction. Western blot assay was carried out to evaluate the protein expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), MMP-2, and MMP-9. Results: The mice treated with atorvastatin have smaller infarct volume and less brain water content than the mice subjected to MCAO/R only. Administration of atorvastatin effectively inhibited cell apoptosis via up-regulation of Bcl-2 protein and down-regulation of Bax protein. In addition, atorvastatin treatment markedly reduced the mRNA and protein expression levels of MMP-2 and MMP-9. Conclusion: Atorvastatin has a neuroprotection effect by means of up-regulating the expression of Bcl-2 and down-regulating the expression of Bax, MMP-2, and MMP-9. Therefore, atorvastatin might be a promising agent for cerebral I/R injury.
- 出版日期2018
- 单位武汉大学
