A Comprehensive Proteome of Mycoplasma genitalium

作者：Parraga Nino Noemi; Colome Calls Nuria; C****s Francesc; Querol Enrique; Ferrer Navarro Mario^*

来源：Journal of Proteome Research, 2012, 11(6): 3305-3316.

DOI：10.1021/pr300084c

• Mycoplasma genitalium
• total proteome
• membrane proteome
• surfome
• immunoproteome

Mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. Proteomic technologies, along with other methods for global gene expression analysis, play a key role in understanding the mechanisms of bacterial pathogenesis and physiology. The proteome of M. genitalium, model of a minimal cell, has been extended using a combination of different proteomic approaches and technologies. The total proteome of this microorganism has been analyzed using gel-based and gel-free approaches, achieving the identification of 85.3% of the predicted ORFs. In addition, a comprehensive analysis of membrane subproteome has been performed. For this purpose, the TX-114 soluble fraction has been analyzed as well as the surface proteins, using cell-surface protein labeling with CyDye. Finally, the serological response of M. genitalium-infected patients and healthy donors has been analyzed to identify proteins that trigger immunological response. Here, we present the most extensive M. genitalium proteome analysis (85.3% of predicted ORFs), a comprehensive M. genitalium membrane analysis, and a study of the human serological response to M. genitalium.

• 出版日期2012-6