Detection of microRNAs in dried serum blots

作者：Patnaik Santosh Kumar^*; Mallick Reema; Yendamuri Sai

来源：Analytical Biochemistry, 2010, 407(1): 147-149.

DOI：10.1016/j.ab.2010.08.004

We observed the preservation of microRNAs in unrefrigerated dried serum blots. Preservation was not adversely affected by drying or storing at 37, 45, or 60 degrees C instead of room temperature, but it was harmed when blots were dried incompletely before storage. Preservation of microRNAs in serum was not diminished if, instead of being kept frozen at -80 degrees C, it was stored as dried blots at room temperature for 5 months or at 37 degrees C for 4 weeks. Thus, dried blots can be a convenient and safer way to save, transport, and store serum for microRNA assays. Published by Elsevier Inc.

• 出版日期2010-12-1