ATP is released by monocytes stimulated with pathogen-sensing receptor ligands and induces IL-1 beta and IL-18 secretion in an autocrine way

作者：Piccini Alessandra^*; Carta Sonia; Tassi Sara; Lasiglie Denise; Fossati Gianluca; Rubartelli Anna

来源：Proceedings of the National Academy of Sciences, 2008, 105(23): 8067-8072.

DOI：10.1073/pnas.0709684105

• inflammation
• nonclassical secretion
• pathogen-associated molecular patterns
• processing

IL-1 beta and IL-18 are crucial mediators of inflammation, and a defective control of their release may cause serious diseases. Yet, the mechanisms regulating IL-1 beta and IL-18 secretion are partially undefined. Both cytokines are produced as inactive cytoplasmic precursors. Processing to the active form is mediated by caspase-1, which is in turn activated by the multiprotein complex inflammasome. Here, we show that in primary human monocytes microbial components acting on different pathogen-sensing receptors and the danger-associated molecule uric acid are all competent to induce maturation and secretion of IL-1 beta and IL-18 through a process that involves as a first event the extracellular release of endogenous ATP. ATP release is followed by autocrine stimulation of the purinergic receptors P2X(7). Indeed, antagonists of the P2X(7) receptor (MA, or treatment with apyrase, prevent IL-1 beta and IL-18 maturation and secretion triggered by the different stimuli. At variance, blocking P2X(7)R activity has no effects on IL-1 beta secretion by monocytes carrying a mutated inflammasome that does not require exogenous ATP for activation. P2X(7)R engagement is followed by K+ efflux and activation of phospholipase A(2). Both events are required for processing and secretion induced by all of the stimuli. Thus, stimuli acting on different pathogen-sensing receptors converge on a common pathway where ATP externalization is the first step in the cascade of events leading to inflammasome activation and IL-1 beta and IL-18 secretion.

• 出版日期2008-6-10