科研之友
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摘要
Thiolated hyaluronic acid (HA) derivatives have been widely studied for their promising applications in tissue engineering and regenerative medicine because of their unique in situ gelation performance, improved degradation behavior, facile bioactive modification, and so on so forth. In this study, the direct thiolated process of hyaluronic acid was improved by optimizing the activities of activating agents of carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) and adjusting the amount of reactants to control the degrees of thiol substitution ranging from 7% to 74%. Thiolated HA (HA-SH) would undergo gelation through partial oxidation of thiol groups in air to disulfides with different mechanical properties corresponding to diverse degree of substitution. Further oxidation using H2O2 facilitated the formation of much firmer hydrogel with high rheological properties and long-term bio-stability in simulated body fluid (SBF).
