Dessert and crab apple seeds as a promising and rich source of all four homologues of tocopherol (alpha, beta, gamma and delta)

作者：Gornas Pawel^*; Seglina Dalija; Lacis Gunars; Pugajeva Iveta

来源：LEBENSMITTEL-WISSENSCHAFT UND-TECHNOLOGIE-FOOD SCIENCE AND TECHNOLOGY, 2014, 59(1): 211-214.

DOI：10.1016/j.lwt.2014.05.006

• Apple seeds
• By-products
• Tocopherols
• RP-HPLC
• UPLC-ESI/MSn

Tocochromanols composition in apple seeds, obtained as a by-product during the fruit salad and juice production, of twelve varieties - seven crab apple ('Kerr', 'Kuku', 'Quaker Beauty', 'Riku', 'Ritika', 'Ruti' and K-8/9-24) and five dessert apples ('Antey, 'Reforest', 'Kent', 'Sinap Orlovskij' and 'Zarja Alatau') were studied. Tocopherols and tocotrienols were isolated using the micro-saponification method of high precision and accuracy and they were analysedanalysed by rapid RP-HPLC/FLD and RP-UPLC-ESI/MSn. Four tocopherols, with predominance of homologues alpha and beta, were detected in each tested sample. The seeds from two apple cultivars 'Antej' and 'Reforest' were characterized by unique and similar ratios of all four tocopherol homologues alpha:beta:gamma:delta (1.7:1.5:1.3:1.0 and 2.1:2.0:1.3:1.0, respectively). The concentration range of individual tocopherol homologues (alpha, beta, gamma and delta) in apple seeds were as follows: 17.22-25.79, 7.53-29.05, 0.61-13.82 and 0.16-10.79 mg/100 g dry weight basis (dwb), respectively. Moreover, three tocotrienols (alpha, beta and gamma) were identified in lesser amounts (0.02-0.74 mg/100 g dwb). Use of apple seeds to isolate tocopherol homologues can ensure better environmental sustainability and effective use of natural plant material.

• 出版日期2014-11