A rare castration-resistant progenitor cell population is highly enriched in Pten-null prostate tumours

作者：Sala Lucila Sackmann; Boutillon Florence; Menara Giulia; De Goyon Pelard Andrea; Leprevost Mylene; Codzamanian Julie; Lister Natalie; Pencik Jan; Clark Ashlee; Cagnard Nicolas; Bole Feysot Christine; Moriggl Richard; Ri**ridger Gail P; Taylor Renea A; Kenner Lukas; Guidotti Jacques Emmanuel; Goffin Vincent^*

来源：Journal of Pathology, 2017, 243(1): 51-64.

DOI：10.1002/path.4924

• prostate cancer
• castration resistance
• epithelial cells
• progenitor cell
• androgen signalling
• CK4

Castration-resistant prostate cancer is a lethal disease. The cell type(s) that survive androgen deprivation remain poorly described, despite global efforts to understand the various mechanisms of therapy resistance. We recently identified in wild-type (WT) mouse prostates a rare population of luminal progenitor cells that we called LSCmed according to their FACS profile (Lin(-)/Sca-1(+)/CD49f(med)). Here, we investigated the prevalence and castration resistance of LSCmed in various mouse models of prostate tumourigenesis (Pb-PRL, Pten(pc-/-), and Hi-Myc mice). LSCmed prevalence is low (similar to 8%, similar to WT) in Hi-Myc mice, where prostatic androgen receptor signalling is unaltered, but is significantly higher in the two other models, where androgen receptor signalling is decreased, rising up to more than 80% in Pten(pc-/-) prostates. LSCmed tolerate androgen deprivation and persist or are enriched 2-3weeks after castration. The tumour-initiating properties of LSCmed from Pten(pc-/-) mice were demonstrated by regeneration of tumours in vivo. Transcriptomic analysis revealed that LSCmed represent a unique cell entity as their gene expression profile is different from luminal and basal/stem cells, but shares markers of each. Their intrinsic androgen signalling is markedly decreased, explaining why LSCmed tolerate androgen deprivation. This also illuminates why Pten(pc-/-) tumours are castration-resistant since LSCmed represent the most prevalent cell type in this model. We validated CK4 as a specific marker for LSCmed on sorted cells and prostate tissues by immunostaining, allowing for the detection of LSCmed in various mouse prostate specimens. In castrated Pten(pc-/-) prostates, there was significant proliferation of CK4(+) cells, further demonstrating their key role in castration-resistant prostate cancer progression. Taken together, this study identifies LSCmed as a probable source of prostate cancer relapse after androgen deprivation and as a new therapeutic target for the prevention of castrate-resistant prostate cancer.

• 出版日期2017-9