An ECL biosensor for sensitive detection of concanavalin A based on the ECL quenching of Ru complex by MoS2 nanoflower

作者：Wang, Bingxin; Zhong, Xia^*; Chai, Yaqin; Yuan, Ruo^*

来源：Sensors and Actuators B: Chemical , 2017, 245: 247-255.

DOI：10.1016/j.snb.2017.01.180

• Concanavalin A
• MoS2 nanoflower
• Ru(dcbpy)(3)(2+)
• Sandwich biosensor

A novel signal-off electrochemiluminescence (ECL) biosensor was constructed for concanavalin A (Con A) detection based on the ECL quenching of Ru complex by MoS2 nanoflower (MoS2 NF). In brief, PTCA-Ru-Au NPs as matrix was dropped on the electrode to immobilize phenoxy dextran (DexP) through pi-pi interaction, where 3,4,9,10-perylenetetracarboxylic acid (PTCA) and Au nanoparticles (Au NPs) could greatly increase the luminous efficiency of Tris (2,2'-bipyridyl-4,4'-dicarboxylato) ruthenium(II) (Ru(dcbpy)(3)(2+)). Then, DexP as recognition element was bonded with Con A via specific carbohydrate-Con A interaction. Finally, the MoS2 NF-multi-walled carbon nanotubes (MWCNT)-DexP as quenching probe of ECL signal was combined with Con A to successfully fabricate a sandwich type ECL biosensor. Importantly, MoS2 NF as a new ECL quencher showed high ECL quenching efficiency toward Ru complex. Under the optimum detection conditions, a wide liner range of 1.0 pg/mL to 100 ng/mL was achieved with relatively low detection limit of 0.3 pg/mL.

• 出版日期2017-6
• 单位西南大学