Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum

作者：Jang Kyoung Soon; Baik Jung Eun; Han Seung Hyun; Chung Dae Kyun; Kim Byung Gee^*

来源：Biochemical and Biophysical Research Communications, 2011, 407(4): 823-830.

DOI：10.1016/j.bbrc.2011.03.107

• Lipoteichoic acid
• Lactobacillus plantarum
• Anti-inflammatory
• NMR
• MALDI-TOF/TOF

Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory properties and is less inflammatory than LTAs from pathogenic bacteria, little is known about the structure of pLTA. In this study, high-field NMR spectra of the pLTA were compared with those of LTA from pathogenic bacterium, Staphylococcus aureus (aLTA). The 2D NMR results demonstrated that pLTA possesses alpha-linked hexose sugar substituents on the poly-glycerophosphate backbone instead of N-acetyl-glucosamine substituents, and unsaturated fatty acids in its glycolipids. The sugar substituents were revealed as an approximately 29:1 molar ratio of the glucose to galactose by HPAEC-PAD analysis. MALDI-TOF/TOF MS analyses identified the presence of unsaturated fatty acids in the glycolipid moieties of pLTA. In addition, the glycolipid structure was found to be composed of trihexosyl-diacyl- and/or trihexosyl-triacyl-glycerol ceramide units by means of unique fragment ions of the glycolipids. These results enabled us to elucidate the pLTA structure, which is distinctively different from canonical LTA structure, and suggest that the unique immunological property of pLTA might be caused by the pLTA structure.

• 出版日期2011-4-22