A generic surface chemistry for peptide microarrays implementation: Application to the detection of anti-H3 antibody

作者：El Khoury Graziella; Laurenceau Emmanuelle^*; Chevolot Yann; Souteyrand Eliane; Cloarec Jean Pierre

来源：Biosensors and Bioelectronics, 2010, 26(4): 1320-1325.

DOI：10.1016/j.bios.2010.07.032

• Surface functionalization
• On-chip peptide synthesis
• Peptide arrays
• Immunoassay
• Antibody-antigen interaction
• Combinatorial peptide synthesis

Peptide microarray can be implemented by immobilization of peptides on a solid support or by direct on-chip peptide synthesis (OCPS). In the first case, peptide primary sequences can be ensured prior to their immobilization but structural diversity is achieved at high cost in terms of reagents. In the second case, high diversity is achieved with low amount of reagents but the primary and secondary structures cannot be ascertained. In both cases, the immobilization step will influence the overall biological activity. We proposed a strategy where direct peptide on-chip synthesis and peptide immobilization are viewed as complementary approaches. In a first step, OCPS is envisioned for the screening and selection of biologically relevant peptides. In a second step, selected peptides would be synthesized on resin, qualified and immobilized for implementing microarrays (i.e. for diagnosis). A versatile surface chemistry for both OCPS and peptide immobilization was developed allowing for an identical physico-chemical environment for both implementation strategies. In the present report, a 16 mer peptide corresponding to the human histone H3 epitope was synthesized on an amino-functionalized support. Surface stability (including upon deprotection steps) and peptide primary and secondary structures were assessed with Cy3-streptavidine conjugates and with immunonassays. Peptides, either on-chip synthesized or immobilized, exhibited a similar biological activity.

• 出版日期2010-12-15