Mast cell-derived mediators promote murine neutrophil effector functions

作者：Doener Fatma; Michel Anastasija; Reuter Sebastian; Friedrich Pamela; Boehm Livia; Relle Manfred; Codarri Laura; Tenzer Stefan; Klein Matthias; Bopp Tobias; Schmitt Edgar; Schild Hansjoerg; Radsak Markus Philipp; Taube Christian; Stassen Michael^*; Becker Marc

来源：International Immunology, 2013, 25(10): 553-561.

DOI：10.1093/intimm/dxt019

• cell activation
• inflammation
• lung
• mast cells
• neutrophils
• rodent

Mast cells are able to trigger life-saving immune responses in murine models for acute inflammation. In such settings, several lines of evidence indicate that the rapid and protective recruitment of neutrophils initiated by the release of mast cell-derived pro-inflammatory mediators is a key element of innate immunity. Herein, we investigate the impact of mast cells on critical parameters of neutrophil effector function. In the presence of activated murine bone marrow-derived mast cells, neutrophils freshly isolated from bone marrow rapidly lose expression of CD62L and up-regulate CD11b, the latter being partly driven by mast cell-derived TNF and GM-CSF. Mast cells also strongly enhance neutrophil phagocytosis and generation of reactive oxygen species. All these phenomena partly depend on mast cell-derived TNF and to a greater extend on GM-CSF. Furthermore, spontaneous apoptosis of neutrophils is greatly diminished due to the ability of mast cells to deliver antiapoptotic GM-CSF. Finally, we show in a murine model for acute lung inflammation that neutrophil phagocytosis is impaired in mast cell-deficient Kit (W-sh)/Kit (W-sh) mice but can be restored upon mast cell engraftment. Thus, a previously underrated feature of mast cells is their ability to boost neutrophil effector functions in immune responses.

• 出版日期2013-10