Direct Effects, Compensation, and Recovery in Female Fathead Minnows Exposed to a Model Aromatase Inhibitor

作者：Villeneuve Daniel L^*; Mueller Nathaniel D; Martinovic Dalma; Makynen Elizabeth A; Kahl Michael D; Jensen Kathleen M; Durhan Elizabeth J; Cavallin Jenna E; Bencic David; Ankley Gerald T

来源：Environmental Health Perspectives, 2009, 117(4): 624-631.

DOI：10.1289/ehp.11891

• adaptation
• endocrine disruption
• estradiol
• fadrozole
• fish
• gene expression
• reproduction
• steroid biosynthesis
• vitellogenin

BACKGROUND: Several chemicals in the environment have the potential to inhibit aromatase, an enzyme critical to estrogen synthesis.
OBJECTIVES: The objective of this study was to provide a detailed characterization of molecular and biochemical responses of female fathead minnows to a model aromatase inhibitor, fadrozole (FAD).
METHODS: Fish here exposed via water to 0, 3, or 30 mu g FAD/L for 8 days and then held in clean water for 8 days, with samples collected at four time points during each 8-day period. We quantified ex vivo steroid production, plasma steroids, and plasma vitellogenin (Vtg) concentrations and analyzed relative transcript abundance of 10 key regulatory genes in ovaries and 3 in pituitary tissue by real-time polymerase chain reaction.
RESULTS: Ex vivo 17 beta-estradiol (E-2) production and plasma E-2 and Vtg concentrations were significantly reduced after a single day of exposure to 3 mu g or 30 mu g FAD/L. However, plasma E-2 concentrations recovered by the eighth day of exposure in the 3-mu g/L group and within 1 day of cessation of exposure in the 30-mu g/L group, indicating concentration- and time-dependent physiologic compensation and recovery. Concentration-dependent increases in transcripts coding for aromatase (A isoform), cytochrome P450 side-chain cleavage, steroidogenic acute regulator), protein, and follicle-stimulating hormone receptor all coincided with increased E-2 production and recovery of plasma E-2 concentrations.
CONCLUSIONS: Results of this research highlight the need to consider compensation/adaptation and recovery when developing and interpreting short-term bioassays or biomarkers or when trying to predict the effects of chemical exposures based on mode of action.

• 出版日期2009-4