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摘要
A rapid and accurate HPLC method has been developed for the simultaneous determination of nine major flavonoids, namely 3 '-hydroxymelanettin, melanettin, stevenin, butein, isoliquiritigenin, dalbergin, 2,4-dihydroxy-5-methoxybenzophenone, pinocembrin, 4-methoxydal-bergione in Dalbergia odorifera. The samples were separated on an Aglient Zorbax SB-C-18 column (250 x 4.6 mm, 5 mu m) with a gradient of acetonitrile and 1 % aqueous acetic acid (v/v) at a flow rate of 1.0 mL min(-1) and detected at 350 nm. The complete separation was obtained within 30 min for the nine target flavonoids. All calibration curves showed good linearity (r(2) > 0.999) within test ranges. The repeatability was evaluated by intra- and inter-day assays and RSD values were less than 4.0%. The recoveries were between 92.0% and 104.5%. The developed method was successfully applied to the analysis of 32 commercial samples of D. odorifera.
