Hydrogen-rich saline improves non-alcoholic fatty liver disease by alleviating oxidative stress and activating hepatic PPAR alpha and PPAR gamma

作者：Zhai Xiao; Chen Xiao; Lu Jiancan; Zhang Yaping; Sun Xuejun; Huang Qin^*; Wang Qijin^*

来源：Molecular Medicine Reports, 2017, 15(3): 1305-1312.

DOI：10.3892/mmr.2017.6120

• non-alcoholic fatty liver disease
• oxidative stress
• inflammation
• hydrogen
• peroxisome proliferator-activated receptor

Non-alcoholic fatty liver disease (NAFLD) comprises a range of liver diseases, between steatosis and non-alcoholic steatohepatitis and liver cirrhosis, which are closely associated with diabetes mellitus. Previous studies have indicated that oxidative stress is a key factor in the development of NAFLD. Molecular hydrogen (H-2) may ameliorate oxidative stress injuries by selectively neutralizing peroxynitrite and hydroxyl radicals. The present study evaluated the effects of H-2 on NAFLD in rats and concluded that H-2-rich saline had significant therapeutic effects on NAFLD induced by hyperglycemia and hyperlipidemia, as demonstrated by hematoxylin and eosin and terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling staining. H-2-rich saline improved fasting blood glucose, fasting insulin, insulin sensitivity and glucose tolerance, and lowered the expression levels of tumor necrosis factor alpha, interleukin-1 beta, 3-nitrotyrosine and 8-hydroxy-2'-deoxyguanosine in the liver. "In addition, the present study revealed that H-2 -rich saline could significantly increase peroxisome proliferator-activated receptor (PPAR) alpha and PPAR gamma expression in hepatocytes. In conclusion, H-2-rich saline may significantly improve NAFLD, possibly by reducing oxidative stress and activating hepatic PPAR alpha and PPAR gamma expression.

• 出版日期2017-3
• 单位中国人民解放军第二军医大学