Drug-liposome distribution phenomena studied by capillary electrophoresis-frontal analysis

作者：Ostergaard Jesper^*; Jorgensen Lene; Thomsen Anne Engelbrecht; Larsen Susan Weng; Larsen Claus; Jensen Henrik

来源：Electrophoresis, 2008, 29(16): 3320-3324.

DOI：10.1002/elps.200700757

• affinity capillary electrophoresis
• distribution coefficient
• frontal analysis
• lipophilicity
• liposome

The potential of using CE frontal analysis (CE-FA) for the study of low-molecular-weight drug-liposome interactions was assessed. The interaction of bupivacaine, bromphenir amine, chlorpromazine, imipramine, and ropivacaine with net negatively charged 80/ 20 mol% 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine/egg yolk phosphatidic acid liposome suspensions in HEPES buffer at pH 7.4 was investigated. The fraction of free drug as a function of lipid concentration was measured and apparent liposome - buffer distribution coefficients were determined for the basic drug substances. The distribution coefficients increased in the order ropivacaine, bupivacaine, brompheniramine, imipramine, and chlorpromazine. The developed CE method was relatively fast allowing estimates of drug-liposome affinity to be obtained within 15 min. CE-FA may have the potential to become a valuable tool for the characterization of drug-liposome interactions in relation to estimation of drug lipophilicity and for the evaluation of drug distribution in liposomal drug delivery systems.

• 出版日期2008-8