Determination of Metformin Hydrochloride and Glyburide in an Antihyperglycemic Binary Mixture Using High-Performance Liquid Chromatographic-UV and Spectrometric Methods

作者：Salem Hesham^*

来源：Journal of AOAC International, 2010, 93(1): 133-140.

Three methods were developed for simultaneous determination of metformin hydrochloride and glyburide in an antihyperglycemic binary mixture without previous separation. In the first method, a reversed-phase HPLC column with acetonitrile-water (60 + 40, v/v) mobile phase at 0.9 mL/min flow rate was used to separate both compounds, with UV detection at 254 nm. Linearity was obtained in the concentration range of 0.06-0.24 mu g/mL for glyburide and 1.5-6.0 mu g/mL for metformin hydrochloride. The second method depended on first- and second-derivative UV spectrometry with zero-crossing measurements. The first-derivative amplitude at 261 nm was selected for the assay of glyburide, and the second-derivative amplitude at 235 nm was selected for the assay of metformin hydrochloride. The third method depended on measuring the first derivative of the ratio-spectra at 241 nm for glyburide and 227 nm for metformin hydrochloride. For the second and third methods, Beer's law was obeyed in the range of 10-55 mu g/mL for glyburide and 20-200 mu g/mL for metformin. The proposed methods were extensively validated and applied for the analysis of some pharmaceutical formulations containing binary mixtures of the mentioned drugs.

• 出版日期2010-2