A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury

作者：East Emma; Johns Noemie; Georgiou Melanie; Golding Jon P; Loughlin A Jane; Kingham Paul J; Phillips James B^*

来源：Regenerative Medicine, 2013, 8(6): 739-746.

DOI：10.2217/rme.13.61

• 3D cell culture
• astrocytes
• CNS
• reactive gliosis
• spinal cord
• stem cell therapy

Aim: This study aimed to develop a 3D culture model to test the extent to which transplanted stem cells modulate astrocyte reactivity, where exacerbated glial cell activation could be detrimental to CNS repair success. Materials %26 methods: The reactivity of rat astrocytes to bone marrow mesenchymal stem cells, neural crest stem cells (NCSCs) and differentiated adipose-derived stem cells was assessed after 5 days. Schwann cells were used as a positive control. Results: NCSCs and differentiated Schwann cell-like adipose-derived stem cells did not increase astrocyte reactivity. Highly reactive responses to bone marrow mesenchymal stem cells and Schwann cells were equivalent. Conclusion: This approach can screen therapeutic cells prior to in vivo testing, allowing cells likely to trigger a substantial astrocyte response to be identified at an early stage. NCSCs and differentiated Schwann cell-like adipose-derived stem cells may be useful in treating CNS damage without increasing astrogliosis.

• 出版日期2013-11