科研之友
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摘要
The present study was aimed to determine the effect of ultrahigh pressure on the anthocyanin content and antioxidant activity of extracts from Lonicera caerulea berries. Response surface analysis predicted that the optimal conditions for ultrahigh-pressure extraction (UPE) included an extraction time of 7 min 43 sec, berry. Extraction buffer ratio of 1: 14.70 (g:mL), and extraction pressure of 426 MPa showed that these conditions would yield a maximum anthocyanin content of 323.85 mg/100 g berries, which was significantly higher than those of extracts produced by ultrasound-assisted extraction and conventional solvent extraction (P < 0.05). In addition, radical scavenging assays indicated that the UPE extracts also exhibited higher antioxidant activity (P < 0.05), and HPLC-DAD-MS indicated that the UPE extracts had higher total anthocyanin contents and higher contents of two potent antioxidants, 5-methylpyranocyanidin-3-hexoside and cyanidin-3-hexoside-ethyl-catechin (P < 0.05). Therefore, current research indicated that UPE can be used as an effective extraction method for bioactive substances.
