科研之友
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摘要
Background Peripheral skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease (COPD) may be due to the disease per se or as a result of concomitant confounding factors. Although the mechanistic basis for this functional impairment is uncertain, oxidative stress may play a role. The purpose of this study was to investigate whether local oxidative stress is associated with the reduced peripheral skeletal muscle performance in rats with emphysema. @@@ Methods In situ mechanical properties of gastrocnemius were measured in Sprague-Dawley rats 5 months after intratracheal instillation of either elastase (EMP, n=10) or normal saline (CON, n=10). Lipofuscin inclusions, myocyte apoptosis and antioxidant enzyme activities were examined in the gastrocnemius muscle. @@@ Results Lipofuscin inclusions were significantly higher in the gastrocnemius muscle of EMP compared with CON (3.2 +/- 0.4 vs. 1.7 +/- 0.4, P<0.01). The activities of antioxidant enzymes were significantly increased in muscle homogenates of EMP as compared to CON. No significant differences were found in myocyte apoptosis between EMP and CON (1.2 +/- 0.9 vs. 1.0 +/- 0.8, P >0.05). EMP decreased the fatigue endurance of gastrocnemius muscle (half-time to fatigue recovery: (150.0 +/- 55.4) seconds vs. (55.2 +/- 29.3) seconds, P <0.01) and had no effect on maximal tetanic force ((467.4 +/- 36.6) g vs. (493.2 +/- 30.5) g, P >0.05). A significantly positive correlation was found between the level of lipofuscin inclusions and the half-time to fatigue recovery of gastrocnemius muscle in EMP (r=0.664, P <0.05). @@@ Conclusion Local oxidative stress may have important functional consequences for peripheral skeletal muscle in rats with EMP. Chin Med J 2010;123(1):40-44
- 出版日期2010-1-5
- 单位首都医科大学
