A first-generation physiologically based pharmacokinetic (PBPK) model of alpha-tocopherol in human influenza vaccine adjuvant

作者：Tegenge Million A^*; Mitkus Robert J

来源：Regulatory Toxicology and Pharmacology, 2015, 71(3): 353-364.

DOI：10.1016/j.yrtph.2015.02.005

• Alpha-tocopherol
• Emulsion
• Vaccines
• Adjuvants
• AS03 (R)

Alpha (alpha)-tocopherol is a component of a new generation of squalene-containing oil-in-water (SQ/W) emulsion adjuvants that have been licensed for use in certain influenza vaccines. Since regulatory pharmacokinetic studies are not routinely required for influenza vaccines, the in vivo fate of this vaccine constituent is largely unknown. In this study, we constructed a physiologically based pharmacokinetic (PBPK) model for emulsified alpha-tocopherol in human adults and infants. An independent sheep PBPK model was also developed to inform the local preferential lymphatic transfer and for the purpose of model evaluation. The PBPK model predicts that alpha-tocopherol will be removed from the injection site within 24 h and rapidly transfer predominantly into draining lymph nodes. A much lower concentration of alpha-tocopherol was estimated to peak in plasma within 8 h. Any systemically absorbed alpha-tocopherol was predicted to accumulate slowly in adipose tissue, but not in other tissues. Model evaluation and uncertainty analyses indicated acceptable fit, with the fraction of dose taken up into the lymphatics as most influential on plasma concentration. In summary, this study estimates the in vivo fate of alpha-tocopherol in adjuvanted influenza vaccine, may be relevant in explaining its immunodynamics in humans, and informs current regulatory risk-benefit analyses. Published by Elsevier Inc.

• 出版日期2015-4