An experimental hyperlipidemia model with periodontitis in mice

作者：Liu, Xiaoxuan; Wang, Zhiguo; Ji, Qiuxia; Sun, Wendong; Hong, Rundan; Xu, Quanchen^*

来源：International Journal of Clinical and Experimental Pathology, 2018, 11(1): 240-247.

• hyperlipidemia
• periodontitis
• mouse model

Purpose: In many animal models and clinical trials, the relationship between periodontitis and hyperlipidemia is bidirectional and interlinked. In this study, an experimental hyperlipidemia model with periodontitis in mice is introduced. Methods: C57BL/6J mice were assigned into group A and B and Apolipoprotein E-deficient (ApoE(-/-)) mice into group C. After 4 weeks of a high fat diet (HFD), group B and C were ligated on the maxillary second molar tooth, and mice were sacrificed after 8 weeks of the HFD. Levels of lipids, interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha in serum after 0, 4, and 8 weeks were determined. Alveolar bone loss (ABL) was assessed under stereomicroscope. Maxillary bones and atherosclerotic lesion area in the aorta were collected for hematoxylin and eosin (HE) staining. Results: After feeding with a HFD for 4 weeks, group C demonstrated dramatic increases in serum lipid levels. The ABL and levels of IL-6, IL-10, and TNF-alpha in group C was significantly higher than those of group A and B (P<0.05). Atherosclerotic lesions were observed in group C. Conclusions: These data demonstrate that an experimental hyperlipidemia model with periodontitis in mice is successfully established by ligation in ApoE(-/-) mice. This method is economical and time saving, and worthy of more general application.

• 出版日期2018
• 单位青岛大学