Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick

作者：Vella Sarah J; Beattie Patrick; Cademartiri Rebecca; Laromaine Anna; Martinez Andres W; Phillips Scott T; Mirica Katherine A; Whitesides George M^*

来源：Analytical Chemistry, 2012, 84(6): 2883-2891.

DOI：10.1021/ac203434x

This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

• 出版日期2012-3-20