Apoptosis of regulatory T lymphocytes is increased in chronic inflammatory bowel disease and reversed by anti-TNF alpha treatment

作者：Veltkamp Claudia; Anstaett Matthias; Wahl Kristin; Moeller Sarah; Gangl Saskia; Bachmann Oliver; Hardtke Wolenski Matthias; Laenger Florian; Stremmel Wolfgang; Manns Michael P; Schulze Osthoff Klaus; Bantel Heike^*

来源：Gut, 2011, 60(10): 1345-1353.

DOI：10.1136/gut.2010.217117

Background and aims Inappropriate immune responses contribute to the continuous stimulation of the intestinal immune system in chronic inflammatory bowel disease (IBD). Among several pathogenic factors, a numerical deficiency of regulatory T (Treg) cells has been suggested to lead to an insufficient compensation of chronically activated T lymphocytes. This study was conducted to investigate whether increased apoptosis contributes to Treg cell deficiency in IBD and whether successful treatment with antitumour necrosis factor alpha (TNF alpha) is achieved by reducing of Treg cell apoptosis.
Methods Apoptosis of CD4(+) Foxp3(+) Treg cells in tissue sections of patients with active IBD was analysed by immunohistochemistry and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labelling) staining. Apoptosis of peripheral blood CD4(+) CD25(+) Foxp3(+) Treg cells was investigated by flow cytometry and annexin-V staining. In addition, caspase activity and apoptosis were measured in sera of patients with IBD treated with anti-TNF alpha by a luminometric caspase enzyme assay.
Results It is demonstrated that patients with active IBD revealed increased apoptosis of local CD4(+) Foxp3(+) Treg cells in the inflamed mucosa compared with non-inflamed control colon tissue. Moreover, in peripheral blood a reduced frequency and increased apoptosis of Treg cells were found and accompanied by elevated caspase activity in the serum. During anti-TNF alpha treatment, Treg cell apoptosis declined in close correlation with elevated peripheral Treg cell numbers and a decrease of caspase activation and disease activity.
Conclusions These data suggest that increased apoptosis of Treg cells plays a potentially important role in the pathogenesis of IBD and can be reversed by anti-TNF alpha treatment. Measurement of Treg cell apoptosis and serum caspase activity might therefore represent promising tools for monitoring disease activity and treatment response in patients with IBD.

• 出版日期2011-10