A cost-effective and efficient strategy for Illumina sequencing of fungal communities: A case study of beech endophytes identified elevation as main explanatory factor for diversity and community composition

作者：Siddique A B; Unterseher M^*

来源：Fungal Ecology, 2016, 20: 175-185.

DOI：10.1016/j.funeco.2015.12.009

• Fagus sylvatica
• European beech
• Illumina sequencing
• High throughput sequencing
• Metabarcoding
• Elevation
• Environmental change
• Leaf-inhabiting microfungi
• Biodiversity
• Ecology
• Endophytes

We describe an accurate and efficient workflow for highly multiplexed paired-end Illumina sequencing of fungal full-length ITS amplicons. The impact of habitat and substratum conditions on leaf-inhabiting fungal communities was analysed. Fully vital and clearly senescent leaves of European beech (Fagus sylvatica) were sampled along an elevation gradient of about 1000 m in the Bavarian Alps, Germany, in autumn 2013. Surface-sterilised leaves were used for genomicDNA extraction, tagging-by-amplification and high-throughput sequencing. Significant correlation of community composition with elevation was observed. The mycobiome was little affected by the physiological state of the leaves, because only a partial shift of taxonomic composition was observed from vital towards clearly senescent leaves.

• 出版日期2016-4