Assessment of functional recovery and axonal sprouting in oligodendrocyte-myelin glycoprotein (OMgp) null mice after spinal cord injury

作者：Ji Benxiu; Case Lauren C; Liu Kai; Shao Zhaohui; Lee Xinhua; Yang Zhongshu; Wang Joy; Tian Tim; Shulga Morskaya Svetlana; Scott Martin; He Zhigang; Relton Jane K; Mi Sha^*

来源：Molecular and Cellular Neuroscience, 2008, 39(2): 258-267.

DOI：10.1016/j.mcn.2008.07.004

• Myelin
• Axonal regeneration
• Spinal cord injury
• Nogo
• MAG
• OMgp

Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite Outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp oil axon regeneration in vivo, OMgp(-/-) mice oil a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp(-/-) mice oil the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp(-/-) littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT+ descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp(-/-) mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp(-/-) mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal Sprouting following dorsal hemisection.

• 出版日期2008-10
• 单位Biogen Idec