New enzymatic methods for selective assay of L-lysine using an L-lysine specific decarboxylase/oxidase from Burkholderia sp AIU 395

作者：Sugawara Asami; Matsui Daisuke; Yamada Miwa; Asano Yasuhisa; Isobe Kimiyasu^*

来源：Journal of Bioscience and Bioengineering, 2015, 119(3): 369-374.

DOI：10.1016/j.jbiosc.2014.08.013

• L-Lysine
• Cadaverine
• L-Lysine oxidase
• L-Lysine decarboxylase
• Putrescine oxidase
• Burkholderia

We developed new enzymatic methods for the selective assay of L-lysine by utilizing an oxidase reaction and a decarboxylation reaction by the L-lysine-specific decarboxylase/oxidase (L-Lys-DC/OD) from Burkholderia sp. AIU 395. The method utilizing the oxidase reaction of this enzyme was useful for determination of high concentrations of L-lysine. The method utilizing the decarboxylase reaction, which proceeded via the combination of the L-Lys-DC/OD and putrescine oxidase (PUO) from Micrococcus rubens, was effective for determination of low concentrations of L-lysine. Both methods showed good linearity, and neither was affected by other amino acids or amines. In addition, the within-assay and between-assay precisions of both methods were within the allowable range. The coupling of L-Lys-DC/OD with PUO was also useful for the differential assay of L-lysine and cadaverine. These newly developed methods were applied to the assay of L-lysine in biological samples and found to be effective.

• 出版日期2015-3