Hemodynamic effects of atrial natriuretic peptide in ischemia-reperfusion injury that occurs after exercise

作者：Vardar Selma Arzu^*; Palabiyik Orkide; Topuz Ruhan Deniz; Gurel Elif Ezgi; Caliskan Semra; Topcu Ozen Serap; Sut Necdet; Karadag Cetin Hakan

来源：Turkish Journal of Medical Sciences, 2015, 45(2): 298-305.

DOI：10.3906/sag-1307-42

• Atrial natriuretic peptide
• ischemia
• exercise
• cardiac contractility
• isatin

Background/aim: Atrial natriuretic peptide (ANP) is known as a protective agent against ischemia-reperfusion injury for cardiomyocytes. We compared the hemodynamic effects of ANP and isatin, which is known as an ANP receptor blocker, in ischemia followed by reperfusion in exercised rat hearts with nonexercised ones.
Materials and methods: Isolated hearts were perfused in 4 exercised (E) groups after a running protocol for 5 days and 4 nonexercised (NE) groups. In the first protocol, ANP was added to the perfusion solution before ischemia in an E and NE group. In the second protocol, different doses of isatin (0.1, 10, 100 mu M/L) were added to the perfusion solution before ANP in 3 E and 3 NE groups. Left ventricular developed pressure (LVDP) and maximum and minimum rates of change in left ventricular pressure (dP/dtmax and dP/dtmin) were recorded.
Results: Higher LVDP and dP/dtmin values were observed in the E group than the NE group following addition of ANP before ischemia. Values of dP/dtmax were higher in the E group at the first minute of reperfusion period. Hemodynamic difference was not observed between groups given the same amount of isatin before ANP.
Conclusion: This study indicated that higher ANP concentrations before ischemia were more effective on the left ventricle contractility and relaxation functions in the hearts that were exposed to exercise.

• 出版日期2015