Arabidopsis ENOR3 regulates RNAi-mediated antiviral defense

作者：Gao, Hua; Yang, Mai; Yang, Haitao; Qin, Yue; Zhu, Biyun; Xu, Gang; Xie, Chengyuan; Wu, Dewei; Zhang, Xiaolin; Li, Wanxiang; Yan, Jianbin; Song, Susheng; Qi, Tiancong^*; Ding, Shou-Wei^*; Xie, Daoxin^*

来源：Journal of Genetics and Genomics, 2018, 45(1): 33-40.

DOI：10.1016/j.jgg.2017.11.005

• Antiviral defense
• CMV-Delta 2b
• DCL2/4
• ENOR3
• RDR1/6
• RNAi

Viruses can infect host plants to cause severe diseases and substantial agricultural loss, while plants have evolved RNA interference (RNAi) strategy to defend against viral infection. Despite enormous efforts, only a few host proteins in RNAi pathway were shown to mediate antiviral defense, including RNA-dependent RNA polymerase 1 (RDR1), RDR6, DICER-LIKE 2 (DCL2) and DCL4. In this study, we carried out a genetic screen for antiviral factors of RNAi pathway in Arabidopsis rdr6 background via inoculation with a 2b-deficient Cucumber Mosaic Virus (CMV-Delta 2b). We identified a mutant susceptible to CMV-Delta 2b, referred to as enhancer of rdr6 (enor) 3-1 rdr6, and found that ENOR3 encodes a functionally unknown protein with high homology to the mammalian Non Imprinted in Prader-Willi/Angelman (NIPA) magnesium transporters. ENOR3 inhibits accumulation of CMV-Delta 2b and acts additively with RDR1, RDR6, DCL2 and DCL4 in antiviral defense. These results uncover that ENOR3 is a key component in antiviral RNAi pathway, and provide new insights into antiviral immunity.

• 出版日期2018-1-20
• 单位首都师范大学; 清华大学