Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay

作者：Zubair Muhammad^*; Ekholm Anders; Nybom Hilde; Renvert Stefan; Widen Cecilia; Rumpunen Kimmo

来源：Journal of Ethnopharmacology, 2012, 141(3): 825-830.

DOI：10.1016/j.jep.2012.03.016

• Cell migration
• Cell proliferation
• Common plantain
• Plantaginaceae
• Plantamajoside
• Wound healing

Ethnopharmacological relevance: The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago major L. (plantain) on cell proliferation and migration in vitro, as a test for potential wound healing properties. %26lt;br%26gt;Materials and methods: Water and ethanol-based extracts were prepared from Plantago major fresh and dried leaves, and tested in vitro in a scratch assay with oral epithelial cells. %26lt;br%26gt;Results: The scratch assay produced reliable results after 18 h. Most of the tested extracts increased the proliferation/migration of the oral epithelial cells compared to the negative control. A concentration of 1.0 mg/mL (on dry weight basis) appears to be optimal regardless of type of extract, and among the alternatives, 0.1 mg/mL was always better than 10 mg/mL Ethanol-based extracts with a concentration of 10 mg/mL had very detrimental effects on cell proliferation/migration. At the other two concentrations, ethanol-based extracts had the most beneficial effect, followed by water extracts of fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves. %26lt;br%26gt;Conclusions: This study suggests that both the water extracts and the more polyphenol-rich ethanol-based extracts of Plantago major leaves have medicinal properties. Further research is, however, needed to determine what compounds are responsible for the wound healing effects.

• 出版日期2012-6-14