科研之友
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摘要
The objectives of this study were i) to determine the effects of the interactions of water activity, temperature and incubation time on the co-production of AFB(1) and CPA by isolates of Aspergillus flavus with different profile of mycotoxin production and ii) to identify the a(w) and temperature limiting conditions for the production of both mycotoxins. Fungi used in this study were selected because they belonged to different chemotypes: chemotype I (AFB(1)+/CPA+), Ill (AFB(1)+/CPA) and IV (AFB(1)/CPA+), respectively. Two culture media were used; Czapek yeast agar (CYA) and corn extract agar (CEM), at different incubated temperatures (10-40 degrees C) and aw levels (0.80-0.98). AFB(1) and CPA production were analyzed after 7, 14, 21 and 28 days of incubation. Significant differences were observed with respect to mycotoxin production depending on the media evaluated. The AFB(1) production occurred more favorably on CYA while the highest CPA concentrations were recorded on CEM. Within the range of aw evaluated in this study, 0.83 was the limiting level for both toxins production. The optimum conditions for AFB(1) production occurred at 0.96 aw and 30 degrees C after 21 days of incubation, regardless of the media and isolate. Although different amounts of toxins were produced in each medium, the limiting and optimum conditions for their production were similar in both. No differences in the response of the three isolates to the abiotic factors discussed were observed despite belonging to different chemotypes. The determination of the thresholds of mycotoxins co-production, especially in the case of data obtained with the corn extract medium can be useful to avoid the conditions conducive to co-occurrence of these mycotoxins in corn.
- 出版日期2014-4
