An international multicentre study on the allergenic activity of air-oxidized R-limonene

作者：Christensson Johanna Brared^*; Andersen Klaus E; Bruze Magnus; Johansen Jeanne D; Garcia Bravo Begona; Gimenez Arnau Ana; Goh Chee Leok; Nixon Rosemary; White Ian R

来源：Contact Dermatitis, 2013, 68(4): 214-223.

DOI：10.1111/cod.12036

• allergic contact dermatitis
• autoxidation
• CAS 5989-27-5
• dose per unit area
• fragrance allergy
• hydroperoxides
• limonene
• oxidation products
• patch test concentration
• patch testing
• terpenes

Background. Limonene is a common fragrance terpene that, in its pure form, is not allergenic or is a very weak allergen. However, limonene autoxidizes on air exposure, and the oxidation products can cause contact allergy. Oxidized R-limonene has previously been patch tested in multicentre studies, giving 23% positive patch test reactions in consecutive patients. Objectives. To investigate whether oxidized R-limonene 3.0% in petrolatum, with a stable concentration of the main haptens, limonene hydroperoxides (Lim-OOHs), could be a useful tool for the detection of contact allergy in an international setting. Methods. Oxidized R-limonene 3.0% (Lim-OOHs 0.33%) pet. was tested in 2900 consecutive dermatitis patients in Denmark, the United Kingdom, Singapore, Spain, Sweden, and Australia. Results. Overall, 5.2% (range 2.312.1%) of the patients showed a positive patch test reaction to oxidized R-limonene. Doubtful reactions were found in 7.0% of the patients (range 024%). Few irritant reactions were seen. Conclusions. Oxidized R-limonene at 3.0% pet. with a specified content of Lim-OOHs 0.33% is a standardized and useful tool for the detection of contact allergy in dermatitis patients. Many patients showing positive patch test reactions to oxidized R-limonene would not be informed of their fragrance allergy if this specific test had not been performed.

• 出版日期2013-4