Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity

作者：Ben Hsouna Anis; Trigui Mohamed; Culioli Gerald; Blache Yves; Jaoua Samir^*

来源：Food Chemistry, 2011, 125(1): 193-200.

DOI：10.1016/j.foodchem.2010.08.060

• Lawsonia inermis
• Naphthalene glycosides
• Acetophenone glycosides
• 1,2,4-Trihydroxynaphthalene-1-O-beta-D-glucopyranoside
• Antioxidant activities

The antioxidant potential of different fractions of Lawsonia inermis (Lythraceae) was investigated. The n-butanolic fraction showed the highest yield of extraction; it also exhibited a strong antioxidant activity in the DPPH assay and a potent capacity in preventing linoleic acid oxidation. Five phenolic glycosides were identified in this fraction. The structure of a new compound was established as 1,2,4-trihydroxynaphthalene-1-O-beta-D-glucopyranoside. In addition, the known 2,4,6-trihydroxyacetophenone-2-O-beta-D-glucopyranoside was described for the first time in this species. The three other compounds, lalioside (2,3,4, 6-tetrahydroxyacetophenone-2-O-beta-D-glucopyranoside), lawsoniaside (1,2,4-trihydroxynaphthalene-1,4-di-O-beta-D-glucopyranoside) and luteolin-7-O-beta-D-glucopyranoside, have been previously reported in L inermis. The antioxidant activity of these glycosides was evaluated by DPPH and beta-carotene assays, and compared to those of commercial standards. 1,2,4-Trihydroxynaphthalene-1-O-beta-D-glucopyranoside was the most active in the DPPH free-radical scavenging test (EC(50) = 6.5 mu g/ml) and showed a moderate inhibition in the beta-carotene bleaching assay. Chemical components of L inermis have good antioxidant capacities and this species could be used as a potential source of new natural antioxidants.

• 出版日期2011-3-1