A genetically encoded aldehyde for rapid protein labelling

作者：Tuley Alfred; Lee Yan Jiun; Wu Bo; Wang Zhiyong U; Liu Wenshe R^*

来源：Chemical communications, 2014, 50(56): 7424-7426.

DOI：10.1039/c4cc02000f

Using a mutant pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

• 出版日期2014