Association of tumor necrosis factor alpha gene polymorphism G-308A with pseudoexfoliative glaucoma in the Pakistani population

作者：Khan Muhammad Imran; Micheal Shazia; Rana Noreen; Akhtar Farah; den Hollander Anneke I; Ahmed Asifa; Qamar Raheel^*

来源：Molecular Vision, 2009, 15(302): 2861-2867.

Purpose: The purpose of the present study was to determine the role of the tumor necrosis factor alpha (TNF-alpha) gene polymorphism G-308A and total serum immunoglobulin E (TsIgE) levels in the onset of pseudoexfoliation glaucoma (PEXG) in Pakistani patients.
Methods: The TNF-alpha polymorphism G-308A was analyzed in 122 patients with PEXG and 126 healthy unrelated controls by using polymerase chain reaction (PCR) and restriction fragment length polymorphism ( RFLP). TsIgE levels were determined by solid-phase enzyme-linked immunosorbent assay (ELISA).
Results: The AA and GA genotypes were strongly associated with PEXG (p<0.001), with an odds ratio ( OR) of 0.07 (95% confidence interval [CI]=0.02-0.27) and 0.24 ( 95% CI=0.12-0.51), respectively, while the GG genotype was found at a higher frequency in controls as compared to patients (p<0.001) OR=8.95 (95% CI=4.55-17.81). No significant difference was found in TsIgE levels of both patients and controls (p=0.86).
Conclusion: The present study concludes that the TNF-alpha polymorphism G-308A is strongly associated with PEXG. To our knowledge this is the first study in southeast Asia which demonstrates a strong association of a TNF-alpha polymorphism with PEXG.

• 出版日期2009-12-22