A New Technology for Stabilization of Biomolecules in Tissues for Combined Histological and Molecular Analyses

作者：Viertler Christian; Groelz Daniel; Guendisch Sibylle; Kashofer Karl; Reischauer Bilge; Riegman Peter H J; Winther Rosa; Wyrich Ralf; Becker Karl Friedrich; Oelmueller Uwe; Zatloukal Kurt^*

来源：Journal of Molecular Diagnostics, 2012, 14(5): 458-466.

DOI：10.1016/j.jmoldx.2012.05.002

For accurate diagnosis, prediction of outcome, and selection of appropriate therapies, the molecular characterization of human diseases requires analysis of a broad spectrum of altered biomolecules, in addition to morphological features, in affected tissues such as tumors. In a high-throughput screening approach, we have developed the PAXgene Tissue System as a novel tissue stabilization technology. Comprehensive characterization of this technology in stabilized and paraffin-embedded human tissues and comparison with snap-frozen tissues revealed excellent preservation of morphology and antigenicity, as well as outstanding Integrity of nucleic acids (genomic DNA, miRNA, and mRNA) and phosphoproteins. Importantly, PAXgene-fixed, paraffin-embedded tissues provided RNA quantity and quality not only significantly better than that obtained with neutral buffered formalin, but also similar to that from snap-frozen tissue, which currently represents the gold standard for molecular analyses. The PAXgene tissue stabilization system thus opens new opportunities in a variety of molecular diagnostic and research applications in which the collection of snap-frozen tissue is not feasible for medical, logistic, or ethical reasons. Furthermore, this technology allows performing histopathological analyses together with molecular studies in a single sample, which markedly facilitates direct correlation of morphological disease phenotypes with alterations of nucleic acids and other biomolecules. (J Mol Diagn 2012, 14:458-466. http://dx.doi.org/10.1016/j.jmoldx.2012.05.002)

• 出版日期2012-9