Adipocyte aminopeptidases in obesity and fasting

作者：Alponti Rafaela Fadoni; Silveira Paulo Flavio^*

来源：Molecular and Cellular Endocrinology, 2015, 415(C): 24-31.

DOI：10.1016/j.mce.2015.07.032

• Aminopeptidases
• ERAP
• Adipocyte
• Obesity
• Food deprivation

This study checked the existence of a diverse array of aminopeptidase (AP) enzymes in high (HDM) and low (LDM) density microsomal and plasma membrane (MF) fractions from adipocytes of control, monosodium glutamate obese and food deprived rats. Gene expression was detected for ArgAP, AspAP, MetAP, and two AlaAP (APM and PSA). APM and PSA had the highest catalytic efficiency, whereas AspAP the highest affinity. Subcellular distribution of AP activities depended on metabolic status. Comparing catalytic levels, AspAP in HDM, LDM and MF was absent in obese and control under food deprivation; PSA in LDM was 3.5-times higher in obese than in normally fed control and control and obese under food deprivation; MetAP in MF was 4.5-times higher in obese than in food deprived obese. Data show new AP enzymes genetically expressed in subcellular compartments of adipocytes, three of them with altered catalytic levels that respond to whole-body energetic demands.

• 出版日期2015-11-5