Adsorption of the antimicrobial peptide tritrpticin onto solid and liquid surfaces: Ion-specific effects

作者：Salay Luiz C; Petri Denise F S; Nakaie Clovis R; Schreier Shirley

来源：Biophysical Chemistry, 2015, 207: 128-134.

DOI：10.1016/j.bpc.2015.10.004

• Antimicrobial peptides
• Adsorption
• Liquid surface
• Solid surface
• Hofmeister ions
• Biointerfaces

Developing functional biointerfaces is important for technological applications. We investigated the interaction and adsorption of the antimicrobial peptide tritrpticin (VRRFPWWWPFLRR, TRP3) onto solid and liquid surfaces and the influence of ions on these processes by several techniques. Surface tension measurements showed that salt addition to TRP3 solution causes a high decrease of surface tension due to the adsorption of TRP3 at air-liquid surface. Ellipsometry studies show the TRP3 adsorption on silicon surfaces forming nanometric films that are able to further interact with liposomes. Contact angle measurements gave insight on the nature of thin film and its roughness. AFM shows the topology of the film on the solid substrates. In addition, those techniques also showed that anions can act as modulators on adsorption phenomena and are correlated with the Hofmeister series. The findings of the current work are relevant for the development of functional interfaces such as biocidal surfaces.

• 出版日期2015-12