A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate

作者：Rajkumar Rahul; Bhaya Bhavana; Mamilla Divya; Czech Theresa; Kisseih Esther; Saini Arun; Chouthai Nitin^*

来源：International Journal of Developmental Neuroscience, 2018, 65(1): 61-65.

DOI：10.1016/j.ijdevneu.2017.10.001

• Cerebrospinal fluid (CSF)
• Glial cell derived neurotrophic factor (GDNF)
• Prematurity
• Gestational age (GA)
• Intraventricular hemorrhage (IVH)

Background: This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns' umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions.
Methods: Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background.
Results: Cord blood GDNF levels were significantly higher (p = 0.004) in preterm newborns (n = 6) (115.05 +/- 57.17, pg/ml) when compared to term newborns (n = 14) (19.67 +/- 10.67, pg/ml). GDNF levels in CSF trended (p = 0.07) higher in term newborns (n = 10) (19.56 +/- 9.11, pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n = 5) (14.49 +/- 3.53, pg/ml).
Conclusions: GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.

• 出版日期2018-4
• 单位西北大学