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摘要
The purpose of this study was to evaluate the capability of silk fibroin (SF) and tricalcium phosphate-coated silk fibroin (SF-TCP) as a bone defect replacement matrix when grafted in calvarial bone defect of rats in vivo. Total 114 calvarial critical size defects (5.0 mm in diameter) made on 57 adult female Sprague-Dawley rats were used for this study. The defects were treated with (1) no graft material (raw defect), (2) autogenous bone, (3) beta-TCP (25 mu g), (4) SF (10 mu g) and (5) SF-TCP (10 mu g). Mean percentage bone area revealed that the SF-TCP group (37.0 +/- 9.1%) had a significantly higher value than that of the SF group (25.0 +/- 11.0%) at postoperative 4 weeks (P<0.05). For bone-specific ALP activity, the SF (23.63 +/- 17.81 U/L) and SF-TCP (23.08 +/- 3.10 U/L) groups had significantly higher values than those of the TCP group (9.06 +/- 3.45 U/L) at postoperative 4 weeks (P<0.0 5). The SF (16.72 +/- 7.35 U/L) and SF-TCP (13.07 +/- 9.35 U/L) groups had significantly lower values than those of the autogenous bone graft group (32.57 +/- 9.35 U/L, P<0.01) at postoperative 8 weeks. For the histomorphometric analysis, both SF and SF-TCP groups had significantly lower bone length, ratio to total length, bone area and ratio to total area values than those of the autogenous bone graft group at both periods (P<0.01). The SF-TCP group showed, during the early healing phase, less immunoreaction and inflammation than the SF group, and revealed a delayed inflammatory reaction at postoperative 8 weeks. These results showed that surface treatment or composition management of silk fibroin might be required, and silk fibroin would be regarded as a candidate scaffold for tissue engineered bone regeneration.
- 出版日期2010-12
