Dissolved Inorganic Carbon Inventory and CO2 Sequestration in Ureolytic Biocalcification Process with Bacillus Pasteurii

作者：Mahanty Biswanath^*; Kim Subin; Kim Chang Gyun

来源：Geomicrobiology Journal, 2014, 31(2): 145-151.

DOI：10.1080/01490451.2013.819051

• biocalcification
• CO(2)sequestration
• DIC
• ureolysis

The generation and depletion of dissolved inorganic carbon (DIC) in a close-environment ureolytic biocalcification process by Bacillus pasteurii was evaluated. Three experimental sets, each containing 50mM urea, were amended with either 50mM Ca2+ before incubation (set-I) or 100mM Ca2+ after 24-h incubation (set-II) or no Ca2+ addition (urea control). Extent of ureolysis was maximum in urea control set (88%), followed by set-II (66.4%) and set-I (35.2%). Out of total DIC generated from microbial metabolism and ureolysis in set-I (277.6mg/l) and set-II (464.9mg/l), only about 54.1mg/l and 180.1mg/l was precipitated as CaCO3, whereas 189.3mg/l and 231.3mg/l DIC escaped into headspace, respectively. Increased time separation between ureolysis and calcification steps in set-II and higher dosage of Ca2+ resulted in synergistic improvement in DIC capture. In a reusability test, the spent supernatant from set-II could precipitate additional amount CaCO(3)from CO(2)saturated water(,)which was twice as much as that of the fresh media control.

• 出版日期2014-2-7