A new medium for Caenorhabditis elegans toxicology and nanotoxicology studies designed to better reflect natural soil solution conditions

作者：Tyne William; Lofts Stephen; Spurgeon David J^*; Jurkschat Kerstin; Svendsen Claus

来源：Environmental Toxicology and Chemistry, 2013, 32(8): 1711-1717.

DOI：10.1002/etc.2247

• Nanoparticle
• Silver
• Toxicity test
• Ionic strength
• Fulvic acid
• pH

A new toxicity test medium for Caenorhabditis elegans is presented. The test solution is designed to provide a better representation of natural soil pore water conditions than currently available test media. The medium has a composition that can readily be modified to allow for studies of the influences of a range of environmentally relevant parameters on nematode biology and toxicology. Tests conducted in the new medium confirmed that nematodes' reproduction was possible at a range of solution pH levels, offering the potential to conduct toxicity studies under a variety of conditions. A test to establish silver nanoparticle and dissolved silver nitrate toxicity, a study type not feasible in M9 or agar media due to precipitation and nanoparticle agglomeration, indicated lower silver nanoparticle (median effective concentration [EC50] of 6.5mg Ag/L) than silver nitrate (EC50 0.28mg Ag/L) toxicity. Characterization identified stable nanoparticle behavior in the new test medium. Environ Toxicol Chem 2013;32:1711-1717.

• 出版日期2013-8