Nuclear Pore Complex Protein Mediated Nuclear Localization of Dicer Protein in Human Cells

作者：Ando Yoshinari^*; Tomaru Yasuhiro; Morinaga Ayako; Burroughs Alexander Maxwell; Kawaji Hideya; Kubosaki Atsutaka; Kimura Ryuichiro; Tagata Maiko; Ino Yoko; Hirano Hisashi; Chiba Joe; Suzuki Harukazu; Carninci Piero; Hayashizaki Yoshihide

来源：PLos One, 2011, 6(8): e23385.

DOI：10.1371/journal.pone.0023385

Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein.

• 出版日期2011-8-15