Detection of Staphyloccocus aureus by real time fluorescence isothermal amplicication

作者：Liu, Yu-Fei; Ye, Lei; Meng, He-Cheng; Yan, He; Shi, Lei

来源：Modern Food Science and Technology, 2013, 29(9): 2262-2266.

• Diagnostic methods
• Fluorescence technologies
• High sensitivity
• High specificity
• Loop mediated isothermal amplificatoin
• Molecular tools
• Stability tests
• Staphyloccocus aureus

Isothermal real-time fluorescence technology is one of the most novel nucleic acid amplification method. 6 primers targeting the nuc gene of Staphyloccocus aureus was applied to detect S. aureus with ESEQuant tube scanner;Specificity of the primers was examined using stander strains of S. aureusand non-S. aureusstrains. S. aureus isolated from food was detected by LAMP based assay and by real time PCR. The results showed that the real time fluorescence isothermal amplicication had good efficiency, high specificity and high sensitivity (102CFU/mL). 50 Staphyloccocus aureus strains isolated from food samples were selected for stability tests. 50 strains was detected as Staphyloccocus aureuswith LAMP and 50 strains were identified with real time PCR. The results in this study suggested that the ESE tube scanner platform has great potential as a field usable molecular tool for diagnosis of Staphyloccocus aureus. In addition, it provide an alternative diagnostic methods for field set, and for detection in developing area.

• 出版日期2013-9
• 单位华南理工大学